Document Type : Original Article
Authors
- Hamid Tebyanian 1
- Seyed Hanif Mirhosseiny 2
- Omidreza Sarrafi 3
- Ehsan Aliakbari 3
- Mehdi Hassanshahian 4
1 Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran
3 Department of Microbiology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University, Lahijan, Iran
4 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, IR Iran
Abstract
Some properties of compounds in degrading bacteria are required for biodegradation of contaminants to higher performance. Those strains which have a high percentage of these features are more effective at biodegradation. The present experiments were designed to measure these parameters. In this study, measurement of cell surface hydrophobic-degrading bacteria was designed which oil was separated from contaminated soils. Hydrophobic cell surface helps to binding bacteria to hydrocarbons which most of them are hydrophobic. In this experiment, the optical density was measured before and after addition of hexadecane at 600 nm. The highest percentage of Cell surface hydrophobicity (BATH%) of strains were 27,32,24 and 29 by Rhodococcus, Achromobacter, Stenotrophomonas and Tsukamurella, respectively and the growths of hydrocarbon degrading bacteria in hexadecane (1%) contain 1.9, 1.85, 1.4 and 1.12, respectively. It shows that whit increasing the amount of hydrophobic cell levels; consequently, the amount of hexadecane degradation will increase.
Keywords
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