Document Type : Original Article

Authors

1 PhD Student, Food science and Technology, Islamic Azad University, Sabzevar, Iran

2 Professor, Department of Food Science and Technology, Ferdowsi University of Mashhad, Iran

3 Assistant Professor, Institute of Food Science and Technology, Mashhad ACECR, Iran

4 Associate Professor, Department of Food Science and Technology, Ferdowsi University of Mashhad, Iran

Abstract

In view of the increase in Saccharomyces cerevisiae mannan content, the culture condition for S.cerevisiae were optimized in this study. The influence of culture condition such as original pH, inoculum size, and temperature on mannan production were evaluated using Response surface methodology. The mathematical model was established by the quadratic rotary combination design. with the order of effect as follows: temperature > initial pH > inoculation size. The optimized culture condition was pH, 6.6; inoculum size, 4 ml; temperature, 32°C. The maximum mannan production increased to 94.912 ±9.1 mg/100 mL at the optimum culture condition.It was evident that the mannan production was affected significantly by culture condition optimization (p < 0.01). After obtaining the optimal conditions, modified alkaline method was used to purify the extracted Mannan. FT-IR spectra analysis showed that the product was chemically pure mannan, that is to say, it contained no other carbohydrates and proteins.

Keywords

Klis, F. M. cell wall assembly in yeast. Yeast 10: 851-869. (1994) Review:
Aguilar, U. B. and J. M. Francois.A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Lett. Appl. Microb. 37: 268-274. (2003)
Inoue, S. B., N. Takewaki, T. Takasuka, T. Mio, M. Adachi, Y. Fujii, C. Miyamoto, M. Arisawa Y. Furuichi, and T. Watanabe.Characterization and gene cloning of 1, 3-β-D-gluca synthase from Saccharomyces cerevisiae. Eur. J. Biochem. 231: 845-854. (1995)
Izabela, L., M. Maria, C. Peter, M. Eva, T. Nakajima, and C. E. Ballou .Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan. J. Biol. Chem. 249: 7685-7694. (1974)
Ponton, J., M. J. Omaetxebarra, N. Elguezabal, M. Alvarez, and M. D. Moragues. Immunoreactivity of the fungal cell wall. Med. Mycol. 39: 101-110. (2001)
Diaz, S., S. Zinker, and H. J. Ruiz.Alterations in the cell wall of Saccharomyce cerevisiae induced by the alpha sex factor or a mutation in the cell cycle. Antonie van Leeuwenhoek 61: 269-276. (1992)
Stanislaw, B., D. R. Wanda, G. Malgorzata, and W. Piotr. Impact of magnesium an mannose in the cultivation media on the magnesium biosorption, the biomass yield and on the cell wall structure of Candida utilis yeast. Eur. Food Res. Technol. 227: 695-700. (2008)
Odds, F. C. and R. Bernaerts .A new differential isolation medium for presuntive identification of clinically important Candida species. J. Clin. Microbiol. 32: 1923-1929. (1994)
Chi, Z. M. and S. Z. Zhao. Optimization of medium and cultivation conditions for pullulan production by a new pullulan-producing yeast strain. Enzym. Microb. Technol. 33: 206-211. (2003)
Zeng, X. B., H. Y. Wang, L. Y. He, Y. C. Lin, and Z. T. Li. Medium optimization of carbon and nitrogen sources for the production of eucalyptene A and xyloketal A from Xylaria sp. 2508 using response surface methodology. Proces. Biochem. 41: 293-298. (2006)
Hong-Zhi Liu, Qiang Wang, Yuan-Yuan Liu, and Fang Fang, Statistical Optimization of Culture Media and Conditions for Production of Mannan by Saccharomyces cerevisiae, Biotechnology and Bioprocess Engineering, 14: 577-583.(2009)
Dallies, N., J. Francois, and V. Paquet. A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants of Saccharomyces cerevisiae. Yeast 14: 1297-1306. (1998)
Zhang, Y. T. and W. Y. Gu. Determination of Mannose in Yeast by Ultraviole Spectrometry. Food Ferment. Ind. 5: 32-36. (1999)
Montgomery, D. C. Design and analysis of experiments (5th ed.). New York: Wiley. pp. 455–492. (2001).
Little, T. M., & Hills, F. J. Agricultural experimentation design and analysis. New York: John Wiley: pp89-123. (1978).
Mendenhall, W.Introduction to probability and statistics (4th ed.). North Settuate, MA: Duxbury Press pp.235-268. (1975).
Daniel, W. W. Biostatistics: A foundation for analysis in the health sciences (5th ed.). New York: Wiley. (1991).
Kappeli, O., M. Muller, and A. Fiechter Chemical and structural alterations of the cell surface of Candida tropicalis induced by hydrocarbon substrate. J. Bacteriol. 133: 952-958. (1978)
Koch, J. and K. Rademacher. Chemical and enzymatic changes in the cell walls of Candida albicans and Saccharomyces cerevisiae by scanning electron microscopy. Can. J. Microbiol. 26: 965-970. (1980)
Ruiz, T. and L. Rodriguez. Effect of anticalmodulin drugs on the action of yeast α factor pheromone. Arch. Microbiol. 145: 104-106. (1986)
Shen, T. and J. Y. Wang. Biochemistry. Higher Education Press, BJ, China. . p. 255 (1990)
Morten, G. and R. Birgitte. Carbon source-de-pendent transcriptional regulation of the mitochondrial glycerol-2-phosphate dehydrogenase gene, Gut2, from Saccharomyces cerevisiae. Can. J. Microbiol. 46: 1096-1100.(2000)
Feng, Y. Y., Z. M. He, S. L. Ong, J. Hu, Z. Zhang, and W. J. Ng. Optimization of agitation, aeration, and temperature conditions for maximum β-mannanase production. Enzym. Microb. Techonol. 32: 282-289. (2003)
Hanan, M. I., M. W. Y. Wan, A. H. Aidil, M. I. Rosli, H.Othman, and O. Othman Optimization of medium for the production of β-cyclodextrin glucanotransferase using central composite design (CCD). Proces. Biochem.40: 753-758. (2005)
Gang L. Huang., Extraction of Two Active Polysaccharides from the Yeast Cell Wall, Z.Naturforsch C. 2008 Nov-Dec;63(11-12):919-21.