Document Type : Original Article
Authors
1 Department of Physiology, Urmia Branch, Islamic Azad University, Urmia, Iran
2 Department of Animal Science, Faculty of Agriculture, Ankara University, Ankara,
3 Department of Animal Science, Faculty College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Jahad-e-Keshavarzi Organization of Tehran Province, Breeding Station of Zandi Sheep, Pishva-Varamin, Iran
Abstract
Objective: The aim of the present study was to evaluate artificial insemination techniques on reproductive performance in Ghezel ewes synchronized with CIDR during breeding season. Methods: All ewes were treated with controlled internal drug release device (CIDR) inserted into the vagina of the ewes for 14 days. All ewes were treated a single intramuscular (IM) dose of PMSG was injected. In this experiment 120 head ewes divided into four subgroups randomly and experimental groups consist of: control 1, ram mating, n=30; control 2, ram mating plus 550 IU PMSG, n=30; group 1, laparoscopical intrauterine insemination plus 550 IU PMSG, n= 30; group 2, cervical insemination plus 550 IU PMSG, n= 30. Results: In this experiment estrus responses were similar in all groups (control 1, 76.7%; control 2, 93%; group 1, 96%; group 2, 100%). There were no statistically significant differences (P>0.05) between the treatment groups and the control groups for the estrus response. Pregnancy rates were 70%, 90.0%, 83.3% and 60% in control groups 1, 2 and AI groups 1, 2 respectively. Twinning rates (10% to 34.6%) and litter size (1.10 to 1.35) were significantly different in the treatment groups and the control group 1 (P<0.05). As a result, conception rate in the laparoscopical intrauterine insemination was higher than cervical insemination.
Keywords
Akoz, M., Bulbul, M., Ataman, B., and Dere, S. (2006). Induction of multiple births in Akkaraman cross-breed synchronized with short duration and different doses of progesterone treatment combined with PMSG outside the
breeding season. Bulletin of the Veterinary Institute in Pulawy, 50: 97-100.
Anel, L., Kaabia, M., and Abroug, B. (2005). Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay.
Theriogenology, 63:1235ñ1247.
Ataman, MB., Akoz, M., and Akman, O. (20060. Induction of synchronized estrus in Akkaraman cross-bred ewes during breeding and anestrus seasons: the use of shortterm and long-term progesterone treatments. Revue de
Medecine Veterinaire, 50: 257-260.
Boscos, CM., Samartzi, FC., Dellis, S., Rogge, A., Stefanakis, A., and Krambovitis, E. (2002). Use progestagengonadotrophin treatment in estrus synchronization of sheep. Theriogenology, 58: 1261-1272.
Dogan, IZ., Nur, H., Gunay, H., Sagirkaya, M., Soylu, K., and Sonmez, C. (2005). Estrus synchronization during the natural breeding season in Anatolian black does. Journal of Veterinary Medicine - Czech, 50: 33-38.
Donovan, A., Hanrahan, JP., Kummen, E., Duffy, P., and Boland, M.P. (2004). Fertility in the ewe following cervical insemination with fresh or frozen-thawed semen at a natural or synchronised estrus. Animal Reproduction
Science, 84(3-4): 359-368.
Donovan, A., Hanrahan, J.P., Lally, T., Boland, M.P., Byrne, GP., Duffy, P., lonergan, P., and Oneill, D.J. (2001). Artificial insemination for sheep using frozen-thawed semen. Dublin: Faculty of Agriculture, Project ARMIS,
4047:1-43
Ehling, C., Wirth, P., Schindler, L., Hadeler, KG., Dˆpke, HH., Lemme, E., Herrmann, D., and Niemann, H. (2003). Laparoscopical intrauterine insemination with different doses of fresh, conserved and frozen semen for the production of ovine zygotes. Theriogenology, 60: 777-787.
Hashemi, M., Safdarian, M., and Kafi, M. (2006). Estrus response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes. Small Ruminant Research, 65: 279-283.
Horoz, H., Kasikci, G., Ak, K., Aklan, S., and Sonmez, C. (2003). Controlling the breeding season using melatonin and progestagen in Kivircik ewes. Turkish Journal of Veterinary and Animal Sciences, 27: 3.
Ince, D., and Karaca, O. (2009). Effects of oestrus synchronization and various doses of PMSG administration in Chios×Kivircik (F1) sheep on
reproductive performances. Journal of Animal and Veterinary Advances, 8: 1948-1952.
Ivanka, B.R.C., and Pawel, M.B. (2011). A Review of Advances in Artificial Insemination (AI) and Embryo Transfer (ET) in Sheep, with the Special Reference to Hormonal Induction of Cervical Dilation and its Implications for Controlled Animal Reproduction and Surgical Techniques . The Open Reproductive Science Journal, 3:162-175
Kaabi ,M. (2002). Ana¥lisis de factores morfoestructurales, instrumentales y metodolo¥gicos de la inseminacio¥n transcervical en la oveja. Tesis Doctoral,
Facultad de Veterinaria, Universidad de Leo¥n, Spain.
Research, 39: 67-71.
Koyuncu, M., Yerlikaya, H., and Altincekic, S.O. (2008). Effect of injection with dexamethasone and progestagens-PMSG on reproduction and rearing of
lambs in semi-intensive condition. Journal of Biological Sciences, 11: 680-682.
Maxwell, W.M.C., and Hewitt, L.J. (1986). A comparison of vaginal, cervical and intrauterine insemination of sheep. Journal of Agricultural Science, 106: 191-193.
Milczewski, V. (2000). Different ways of sheep artificial insemination using cooled semen. Scientia Agraria, 1:83- 95.
Milczewski, V., Kozicki, L.E., Luz, S.L.N., and Neves, J.P. (2000). Intrauterine and cervical artificial insemination in sheep using cooled semen. Archives of Veterinary Science, 5: 35-39.
Najafi, G., and Cedden, F. (2014). Estrus synchronization and twinning rate of Ghezel ewes treated with CIDR and PMSG during the breeding season. Online Journal of Animal and Feed Research, 4(6).
OíMeara, C.M., Hanrahan, J.P., Donovan, A., Fair, S., Rizos, D., Wade, M., Boland, M.P., Evans, A.C.O., and Lonergan, P. (2005). Relationship between in vitro fertilization of ewe oocytes and the fertility of ewes following cervical
artificial insemination with frozenthawed ram semen. Theriogenology, 64: 1797-1808.
÷zbey, O., and Tatli, P. (20010. ›vesi koyunlarda flushing ve sinkronizasyon uygulamalamm dˆl verimi ¸zerine etkisi. Uludað ‹niversitesi Veteriner Fak¸ltesi Dergisi, 20: 109-116.
Paulenz, H., Adnoy, T., Fossen, O.H., Soderquist, L., and Berg, K.A. (2002). Effect of deposition site and sperm number on the fertility of sheep inseminated with liquid semen. Veterinary Record, 150: 299ñ302.
Salamon, S., and Maxwell, W.M.C. (2000). Storage of ram semen. Animal Reproduction Science, 62: 77ñ111.
Safranski, T.J., Lamberson, W.R., and Keisler, D.H. (1992). Use of melengesterol acetate and gonadotropins to induce fertile estrus in seasonally anestrous ewes. Journal of Animal Science, 70: 2935-2941.
Salehi, R., Kohram, H., Towhidi, A., Kermani, M.H., and Honarvar, M. (2010). Follicular development and ovulation rate following different superovulatory
treatments in Chall ewes. Small Ruminant Research, 93: 213ñ217
Chapman, Hall. (2002). A handbook of statistical analyses using SAS. USA.
Simonetti, L., Ramos, G., and Gardon, J.C. (2002). Effect of estrous synchronization and artificial insemination on reproductive performance of Merino sheep. Brazilian Journal of Veterinary Research and Animal Science, 39:143-146.
Smith, P.A., Boland, M.P., and Gordon, I. (1981). Effect of type of intravaginal progestagen on the outcome of fixedtime artificial insemination. Journal of Agricultural Science, 96: 243- 245.
Timurkan, H., and Yildiz, H. (2005). Synchronization of oestrus in Hamedani ewes: the use of different PMSG dose. Bulletin of the Veterinary Institute in Pulawy, 49: 311-314.
Ustuner, B., Gunay, U., Nur, U., and Ustuner, U. (2007). Effects of long and short-term progestagens treatment combined with PMSG on oestrus synchronization and fertility in Awassi ewes during the breeding season.
Journal of Acta Veterinaria Brno, 76:391-397.
Wildeus, S. (1999). Current concepts in synchronization of estrus: sheep and goats. Journal of Animal Science, 77: 1-14.
Yavuzer, U. (2005). The possibility of the twice-yearly lambing of Awassi sheep ewes without using hormones in an organic animal production system. Turkish Journal of Veterinary and Animal Sciences, 29: 27-30.
Zeleke, M., Greyling, J.P.C., Schwalbach, L.M.J., Muller, T., and Erasmus, J.A. (2005). Effects of PMSG dose on estrous synchronization and fertility in Dorper ewes during transition period. Small Ruminant Research, 56: 47-53.
)