International Journal of Advanced Biological and Biomedical Research

Effects of Fluorescent Quantum Dots on COVID-19 Detection:‎‏ ‏A Survey on Present ‎Findings, Challenges, and Future Viewpoints

Elaheh Farahani; Mozhdeh Haddadi

Volume 10, Issue 4 , October 2022, , Pages 288-298

https://doi.org/10.22034/ijabbr.2022.561871.1417

Abstract

Acute coronavirus syndrome (SARS-CoV-2) leads to the COVID-19 epidemic. In accordance with the WHO, the epidemic remains a serious danger to the general health because of its global ... Read More

Screening of bacteria Streptomyces Waksman and Henrici 1943 (Streptomycetaceae) Isolates from Soil Samples in Iraq

Bassam Qasim; Mohsen Hashim Risan

Volume 9, Issue 4 , October 2021, , Pages 340-351

https://doi.org/10.22034/ijabbr.2021.534456.1364

Abstract

Background: The genus Streptomyces Waksman & Henrici 1943 includes aerobic, gram-positive, and filamentous bacteria which produce well developed vegetative hyphae with branches. ... Read More Background: The genus Streptomyces Waksman & Henrici 1943 includes aerobic, gram-positive, and filamentous bacteria which produce well developed vegetative hyphae with branches. The wall consists of a large mixture of different compounds, including peptidoglycan, teichuronic acid, teichoic, and polysaccharides. The peptidoglycan components consist of glycan as a chains of irregular N-acetyl- d-muramic acid (NAM), diaminopimelic acid, and N-acetyl-d-glucosamine (NAG) and DAP, which is unique in the cell walls of prokaryotic microorganisms. The teichoic and teichuronic acid are chemically bonded to peptidoglycan.Methods: One gram of soil samples was used to make suspension, by adding 99 mL of sterile distilled water (stock suspension) into it and shaking it in a shaker at 160 rpm for 30 minutes at room temperature. Serial dilutions from 0.1-0.001 were made from the stock suspension, and left for 10 minutes. After shaking, 0.1 mL of each dilution was cultured on Yeast Extract and Malt Extract agar (YEME) with Streptomycin 50 ug/mL. The inoculated plates were incubated at 28 °C for 7 to 10 days. Based on cultural characteristics, suspected colonies of Streptomyces were selected, which are characterized as small, white, pin-point, rough, chalky, and a clear zone of inhibition around them. These colonies were confirmed their identification by types of Gram’s stain, aerial and substrate mycelium color, pigment production, and pigment color. Streptomyces were re-streaked on International Streptomyces project (ISP) to obtain pure colonies used for identification. Results: The current study aimed to screen bacteria Streptomyces isolates. Only 21 samples of soil were suspected to contain Streptomyces, and 45 isolates were obtained with different morphology types per samples of soil. The colonies suspects were selected basis on color that ranged from gray, white and creamy. The microscopic examination of local Streptomyces spp. after Gram-staining method was conducted. The observations revealed that local Streptomyces is gram positive and rod shaped similar to features of fungal in possessing branched mycelium. The Streptomyces produced extra cellular enzymes like amylase, urease, catalase, protease, Gelatinase, cellulase and phosphatase. Utilization of citrate was positive, with no Melanine reaction production and soluble pigmented, and negative for indole production.Conclusion: The identification of the Streptomyces is a very complex process. Morphological and biochemical characteristics are two important aspects for the classification in the Streptomycetaceae family. By studying the morphological, cultural, and biochemical characteristics, it is observed that the local isolates are belonging to the genus of Streptomyces.

Evaluation of Relationship between Vitamin D Supplementation Treatment and Hypercalciuria in Patients with Kidney Stones

Elham Javadian; Ali Alidadi; Mahmoud Ali Kaykhaei

Volume 9, Issue 4 , October 2021, , Pages 352-361

https://doi.org/10.22034/ijabbr.2021.537924.1366

Abstract

Background: Vitamin D, especially calcitriol (its active metabolite), increases gastrointestinal calcium absorption. Because urinary calcium excretion is associated with calcium absorption ... Read More

Factors Affecting Level of Knowledge on Hepatitis C among Young Couples before Marriage: A Cross-Sectional Study

Aida Moarrefzadeh; Ali Delpisheh; Arash Sarveazad; Jebreil Shamseddin; Mansour Bhardoust

Volume 9, Issue 4 , October 2021, , Pages 362-371

https://doi.org/10.22034/ijabbr.2021.538010.1367

Abstract

Background: Despite the great importance of public knowledge of sexually transmitted diseases such as hepatitis c virus (HCV), very few studies have been conducted in this field in ... Read More Background: Despite the great importance of public knowledge of sexually transmitted diseases such as hepatitis c virus (HCV), very few studies have been conducted in this field in Iran. Therefore, the present study determined the factors affecting the knowledge of young couples before marriage in relation to HCV.Methods: During this cross-sectional study, 1501 couples were assessed, out of which 1110 (73.9%) couples entered the study. A standard questionnaire was used to collect the data. In this questionnaire, first, the demographic characteristics of the participants such as age, gender, level of education, daily extracurricular study, and hours of mass media use were collected, followed by the main items of the study. After data gathering, they were analyzed by IBM SPSS Statistical Software Version 22. Univariate analysis was used to examine the relationship between factors related to the knowledge, and multivariate analysis was used to estimate the related factors.Results: The total score of knowledge about hepatitis C was 0.49 ± 0.29. The total knowledge score for hepatitis C symptoms was 0.27± 0.19. The total score of knowledge about hepatitis C transmission routes was 0.34±0.28. The univariate analysis showed that age, level of education, history of hepatitis in a first degree relative of the family, and social networks were significantly associated with the rate of knowledge about hepatitis C (p <0.05). The multivariate analysis showed that females [(B: 2.11), CI 95% (1.07_3.25), p: 0.014)], higher education level [(B: 4.01), CI 95% (1.98_6 .33), p: 0.014)], history of hepatitis in a first degree relative of the family [(B: 3.56), 95% CI (1.46_5.69), p: 0.011)] and use of social networks [ (B: 2.77), CI 95% (1.11_4.48), p: 0.014)] were significantly associated with the rate of knowledge about HCV.Conclusion: Compared with other studies in this field, the level of knowledge about hepatitis C among young couples in Iran is alarmingly low. In health policies related to hepatitis C, more attention should be paid to the population of men, people with less than a bachelor's degree.

Survival and Growth Promoting Effects of Bacillus subtilis on Macrobrachium rosenbergii, and Validation of Gut Microflora

Periyakali Saravana Bhavan; Duraisamy Anitha; Thankaraj Manjula; Rajendran Udayasuriyan; Ramasamy Kalpana; Madhayan Karthik

Volume 8, Issue 4 , November and December 2020, , Pages 440-460

https://doi.org/10.22034/ijabbr.2020.43288

Abstract

This work emphasized the survival and growth of the commercially important freshwater prawn, Macrobrachium rosenbergii post-larvae on formulated diets supplemented with a probiotic ... Read More

International Journal of Advanced Biological and Biomedical Research

Articles in Press

Current Issue

Volume 12 (2024)

Volume 11 (2023)

Volume 10 (2022)

Volume 9 (2021)

Volume 8 (2020)

Volume 7 (2019)

Volume 6 (2018)

Volume 5 (2017)

Volume 4 (2016)

Volume 3 (2015)

Volume 2 (2014)

Volume 1 (2013)

Effects of Fluorescent Quantum Dots on COVID-19 Detection:‎‏ ‏A Survey on Present ‎Findings, Challenges, and Future Viewpoints

Abstract

Screening of bacteria Streptomyces Waksman and Henrici 1943 (Streptomycetaceae) Isolates from Soil Samples in Iraq

Abstract

Evaluation of Relationship between Vitamin D Supplementation Treatment and Hypercalciuria in Patients with Kidney Stones

Abstract

Factors Affecting Level of Knowledge on Hepatitis C among Young Couples before Marriage: A Cross-Sectional Study

Abstract

Survival and Growth Promoting Effects of Bacillus subtilis on Macrobrachium rosenbergii, and Validation of Gut Microflora

Abstract